ExtFig3A_RACE_BCAP31_R3_LG293_annotated.tiff
收藏DataCite Commons2023-01-19 更新2024-08-18 收录
下载链接：
https://figshare.com/articles/dataset/ExtFig3A_RACE_BCAP31_R3_LG293_annotated_tiff/21804513/1
下载链接
链接失效反馈官方服务：
资源简介：
<em>Xrn1 knock out A549 cells were infected with WT PR8 or PR8-PA(∆X), or mock infected. 5’ RACE was then performed using primers specific for BCAP31, TUBA1B, INSIG1 or SLC7A5, positioned ~ 200-300 nucleotides downstream of the predicted cut sites.The PCR products were run on an agarose gel. </em>
将Xrn1基因敲除的A549细胞分别用野生型（WT）PR8毒株、PR8-PA(∆X)毒株感染，另设空白感染对照组。随后以针对BCAP31、TUBA1B、INSIG1及SLC7A5的特异性引物开展5' 末端快速扩增（5' RACE）实验，引物位点均位于各靶基因预测剪切位点下游约200~300个核苷酸区域。将所得聚合酶链式反应（Polymerase Chain Reaction, PCR）产物进行琼脂糖凝胶电泳。
提供机构：
figshare
创建时间：
2023-01-19
