Supplementary File 2 - Table 1: Abundance table and taxonomic affiliation of uncharacterized ASVs.

This dataset is additional materials for the study "A FAIR Amplicon Sequencing Workflow for Long-term Environmental Monitoring". This paper describes a new bioinformatic tool dedicated to long-term environmental monitoring of microbial communities. The Reproducible Amplicon Sequencing Pipeline for Antarctic Monitoring (RASPAM) is built on Apptainer containers and Nextflow workflows. RASPAM implements Amplicon Sequence Variants (ASVs) and Zero-radius Operational Taxonomic Units (ZOTUs) to provide high-resolution taxonomic affiliation and sensitive description of microbial communities. It incorporates, in addition to the SILVA database, a taxonomically curated 16S rRNA database for cyanobacteria and enables comparisons against NCBI databases to facilitate the identification of rare prokaryotic strains in environmental samples. RASPAM is Findable, Accessible, Interoperable, and Reproducible (FAIR) and represents a robust tool for long-term monitoring of microbial communities in Antarctic and other extreme environments. The tool is also easily configurable, to adapt to various case studies.
The paper associated to this dataset is accessible on BioRxriv : https://doi.org/10.64898/2025.12.04.692289

本数据集为研究论文《面向长期环境监测的FAIR扩增子测序工作流（A FAIR Amplicon Sequencing Workflow for Long-term Environmental Monitoring）》的配套补充材料。该论文介绍了一款专为微生物群落长期环境监测打造的新型生物信息学工具。南极监测可复用扩增子测序流程（Reproducible Amplicon Sequencing Pipeline for Antarctic Monitoring，简称RASPAM）基于Apptainer容器与Nextflow工作流构建而成。RASPAM采用扩增子序列变异体（Amplicon Sequence Variants，简称ASVs）与零半径操作分类单元（Zero-radius Operational Taxonomic Units，简称ZOTUs）技术，可实现高精度的物种分类注释与高灵敏的微生物群落特征解析。该流程除搭载SILVA数据库外，还集成了一套针对蓝细菌的经分类学手工整理的16S rRNA精选数据库，并支持与NCBI数据库进行比对，以辅助环境样本中稀有原核菌株的识别。RASPAM具备可发现（Findable）、可获取（Accessible）、可互操作（Interoperable）与可复用（Reproducible）四大特性（即FAIR原则），是一款适用于南极及其他极端环境下微生物群落长期监测的可靠工具。该工具还具备灵活的可配置性，可适配各类不同的研究场景。
本数据集关联的论文可在bioRxiv平台获取：https://doi.org/10.64898/2025.12.04.692289