Genome-wide maps of p53 binding sites in human embryonic stem cell H9 derived trilineage.

To determine the roles of IFI16-p53 interaction during H9-trilineage specification, we performed ChIP-seq to derive a genome-wide profile of p53-binding genes after IFI16 knockdown by shRNA-1865. p53 binding sites were mapped on Day 2 of trilineage induction. Overall design: Examination of p53 peaks in 3 cell types after IFI16 knockdown.

为探明IFI16与p53的相互作用在H9细胞三系分化特化过程中的功能，我们采用shRNA-1865敲低IFI16后，通过染色质免疫共沉淀测序（ChIP-seq）构建了全基因组范围的p53结合基因谱。本次实验在三系诱导分化的第2天完成p53结合位点的定位。实验整体设计：对IFI16敲低后的3种细胞类型中的p53结合峰进行检测。