Additional file 2 of Dysregulated lipid metabolism in a retinal pigment epithelial cell model and serum of patients with age-related macular degeneration

Additional file 2: Tables S2–S5. Table S2 Small molecules detected and identified in positive ionization mode. A total of 185 compounds where tentatively identified using ID browser, showing the normalized averaged data for each of the tentatively identified compounds. Table S3 Small molecules detected and identified in negative ionization mode. A total of 68 compounds where tentatively identified using ID browser, showing the normalized averaged data for each of the tentatively identified compounds. Table S4 Demographic characteristics of AMD patients (n = 20) and control subjects (n = 20). Table S5 Comparison of selected lipids levels in serum samples from control (n = 20) and AMD patients (n = 20). Lipid levels were normalized to the sample volume and expressed as the mean ± standard deviation across 20 biological replicates for each group. Group comparisons were conducted using the Mann–Whitney or unpaired t-test, depending on data distribution normality assessed via the Kolmogorov–Smirnov test. To account for multiple comparisons, p values were adjusted using the Benjamini–Hochberg method. Statistical significance is indicated as follows: ns: adjusted p value > 0.05; *: adjusted p value < 0.05; **: adjusted p value < 0.01; ***: adjusted p value < 0.001.

附加文件2：表S2~S5。表S2：正离子模式下检测并鉴定的小分子。共通过ID浏览器（ID browser）初步鉴定出185种化合物，展示了每种初步鉴定化合物的归一化平均数据。表S3：负离子模式下检测并鉴定的小分子。共通过ID浏览器（ID browser）初步鉴定出68种化合物，展示了每种初步鉴定化合物的归一化平均数据。表S4：年龄相关性黄斑变性（AMD）患者（n=20）与对照受试者（n=20）的人口统计学特征。表S5：对照组（n=20）与AMD患者（n=20）血清样本中选定脂质水平的比较。脂质水平已按样本体积进行归一化，以每组20个生物学重复的平均值±标准差（mean ± standard deviation）表示。根据通过柯尔莫哥洛夫-斯米尔诺夫检验（Kolmogorov–Smirnov test）评估的数据分布正态性，组间比较采用曼-惠特尼检验（Mann–Whitney test）或非配对t检验（unpaired t-test）。为校正多重比较，采用本杰明尼-霍赫贝格方法（Benjamini–Hochberg method）对p值进行校正。统计学显著性标注规则如下：ns：校正后p值>0.05；*：校正后p值<0.05；**：校正后p值<0.01；***：校正后p值<0.001。