Data from: Catecholamine-mediated release of miR-133a-3p from adipocytes regulates the onset of chronic primary pain

Description of methods used for collection/generation of data: Using the TaqMan (R) OpenArray (R) Rodent MicroRNA Panel (ThermoFisher Cat No. 4461105), high-throughput RT-qPCR was performed to measure the expression of microRNAs between experimental groups using the methods recommended by the supplier.

Experimental conditions: 5 female rats per group. 3 groups. Using 2 osmotic mini pumps for subcutaneous drug delivery, rats were either treated with the COMT enzyme inhibitor OR486 or vehicle in one mini pump and the beta-2 and beta-3 adrenergic receptor inhibitors ICI-118,551 and SR 59230A or respective vehicles. The groups represented in the data set are: "vv" = Vehicle/Vehicle, "orv" = OR486/Vehicle, and "oris" = OR486/ICI-118,551 and SR 59230A. Whole blood from rats were collected after 14 days of treatment, then centrifuged to isolate plasma. RNA was isolated from plasma using a RNA isolation from plasma kit (ThermoFisher) before cDNA synthesis. Synthesized cDNA was then used for the standard OpenArray protocol.

Publication under review will list more detailed methods for RNA isolation.

数据采集与生成所用方法说明：本研究采用赛默飞世尔（ThermoFisher）货号为4461105的TaqMan（注册商标）OpenArray（注册商标）啮齿类微小RNA（microRNA）检测板，按照供应商推荐的实验方案，通过高通量实时荧光定量逆转录聚合酶链反应（RT-qPCR）检测各组实验动物间微小RNA的表达水平。

实验设置：每组包含5只雌性大鼠，共设置3组。通过2台渗透性微型植入泵进行皮下给药：大鼠单侧泵植入儿茶酚-O-甲基转移酶（COMT）抑制剂OR486或对应溶媒，另一侧泵植入β2及β3肾上腺素能受体抑制剂ICI-118,551、SR 59230A或对应溶媒。本数据集包含的实验组如下："vv"组为溶媒/溶媒对照组，"orv"组为OR486/溶媒组，"oris"组为OR486联合ICI-118,551与SR 59230A给药组。大鼠经14天给药后采集全血，经离心分离血浆；使用赛默飞世尔血浆RNA提取试剂盒从血浆中提取RNA，随后进行互补脱氧核糖核酸（complementary DNA，cDNA）合成。合成得到的cDNA将按照标准OpenArray实验流程开展后续检测。

待发表的相关论文将详细说明RNA提取的具体实验方法。