Branched chain keto acid dehydrogenase activity in lean and obese Zucker rats*.

*Data from cohort C. Enzyme activities were measured at 30°C. Values are means ± SEM; n = 10 rats per group. NS indicates not significant, P>0.05.aMuscle and adipose tissue BCKDC activities were measured using the 13C method [57], which does not use U indicating µmol of NADH formed. BCKDC total activities were unavailable for muscle; neither total nor actual BCKDC activities were available for adipose tissue. Unfortunately, activation of BCKDH using lambda phosphatase did not work in these experiments. Additionally, the adipose tissue values were very low and too close to the level of detection and contamination from room air and are not reported.

本数据来自队列C。酶活性的测定温度为30℃。数值以平均值±标准误（Standard Error of the Mean，SEM）表示；每组包含10只大鼠。NS代表无统计学显著性（P>0.05）。a：肌肉与脂肪组织的支链α-酮酸脱氢酶复合体（Branched-chain α-keto acid dehydrogenase complex，BCKDC）活性采用13C标记法[57]测定，该方法无需使用U来表示生成的烟酰胺腺嘌呤二核苷酸还原态（Nicotinamide adenine dinucleotide reduced，NADH）的微摩尔数。肌肉组织的BCKDC总活性无法获取；脂肪组织既无法获取总BCKDC活性，也无法获取实际BCKDC活性。遗憾的是，本实验中采用λ磷酸酶激活支链α-酮酸脱氢酶（BCKDH）的操作未获成功。此外，脂肪组织的测定值极低，且与检测限及室内空气的污染水平极为接近，故未予报道。