Table_1_High Levels of HIST1H2BK in Low-Grade Glioma Predicts Poor Prognosis: A Study Using CGGA and TCGA Data.DOCX

A number of biomarkers have been identified for various cancers. However, biomarkers associated with glioma remain largely to be explored. In the current study, we investigated the relationship between the expression and prognostic value of the HIST1H2BK gene in glioma. Sequential data filtering (survival analysis, independent prognostic analysis, ROC curve analysis, and clinical correlation analysis) was performed, which resulted in identification of the association between the HIST1H2BK gene and glioma. Then, the HIST1H2BK gene was analyzed using bioinformatics (Kaplan–Meier survival analysis, univariate Cox analysis, multivariate Cox analysis, and ROC curve analysis). The results showed that low expression of HIST1H2BK was associated with better prognosis, and high expression of HIST1H2BK was associated with poor prognosis. In addition, HIST1H2BK was an independent prognostic indicator for patients with glioma. We also evaluated the association between HIST1H2BK and clinical characteristics. Furthermore, gene set enrichment analysis (GSEA) and analysis of immune infiltration were performed. The results showed that HIST1H2BK was associated with intensity of immune infiltration in glioma. Finally, co-expression analysis was performed. The results showed that HIST1H2BK was positively correlated with HIST1H2AG, HIST2H2AA4, HIST1H2BJ, HIST2H2BE, and HIST1H2AC, and negatively correlated with PDZD4, CRY2, GABBR1, rp5-1119a7.17, and KCNJ11. This study showed that upregulation of HIST1H2BK in low-grade glioma (LGG) tissue was an indicator of poor prognosis. Moreover, this study demonstrated that HIST1H2BK may be a promising biomarker for the treatment of LGG.

目前已在多种癌症中鉴定出诸多生物标志物，但与胶质瘤相关的生物标志物仍有大量待探索之处。本研究针对胶质瘤中HIST1H2BK基因的表达水平与预后价值之间的关联展开探究。本研究通过序列数据筛选（包括生存分析、独立预后分析、受试者工作特征（Receiver Operating Characteristic，ROC）曲线分析及临床相关性分析），明确了HIST1H2BK基因与胶质瘤的关联。随后，我们借助生物信息学方法对HIST1H2BK基因展开分析，分析内容涵盖卡普兰-迈耶（Kaplan–Meier）生存分析、单变量考克斯（Cox）回归分析、多变量考克斯（Cox）回归分析及ROC曲线分析。分析结果显示，HIST1H2BK基因低表达与更佳的预后相关，而其高表达则预示不良预后。此外，HIST1H2BK基因可作为胶质瘤患者的独立预后指标。本研究还探究了HIST1H2BK基因与胶质瘤患者临床特征之间的关联。此外，本研究开展了基因集富集分析（Gene Set Enrichment Analysis，GSEA）与免疫浸润分析。结果表明，HIST1H2BK基因与胶质瘤中的免疫浸润强度密切相关。最后，本研究开展了共表达分析。结果显示，HIST1H2BK基因与HIST1H2AG、HIST2H2AA4、HIST1H2BJ、HIST2H2BE及HIST1H2AC呈正相关，而与PDZD4、CRY2、GABBR1、rp5-1119a7.17及KCNJ11呈负相关。本研究证实，低级别胶质瘤（Low-grade Glioma，LGG）组织中HIST1H2BK基因的上调可作为不良预后的指示因子。此外，本研究表明HIST1H2BK基因或可成为低级别胶质瘤治疗领域极具潜力的生物标志物。