A novel simultaneous short-course nitrification, denitrification and fermentation process: bio-enhanced phenol degradation and denitrification in a single reactor

To further analyze the metabolic mechanisms and interactions between functional bacteria in the SNDF system, activated sludge was sampled on day 156 and analyzed using metagenomic sequencing. DNA integrity was detected by gel electrophoresis. DNA extracts were fragmented (~400 bp) using Covaris M220 (Genedenovo Biotechnology, Guangzhou, China) to generate paired-end libraries. Samples were sequenced by Illumina NovaSeq/Hiseq Xten(Illumina, USA) platform (Guangzhou, China). 16sRNA sequencing was used to determine changes in microbial community structure.

为进一步解析SNDF系统中功能菌的代谢机制及其相互作用，研究于第156天采集活性污泥样品，采用宏基因组测序技术开展相关分析。通过凝胶电泳检测DNA完整性。使用Covaris M220（中国广州吉诺德诺生物技术有限公司）对DNA提取物进行片段化处理，片段长度约为400 bp，以构建双端测序文库。样品依托美国Illumina公司的Illumina NovaSeq/Hiseq Xten测序平台（中国广州）完成测序。采用16S核糖体RNA测序（16sRNA sequencing）分析微生物群落结构的变化。