Additional file 1 of Combination of an autophagy inhibitor with immunoadjuvants and an anti-PD-L1 antibody in multifunctional nanoparticles for enhanced breast cancer immunotherapy

Additional file 1: Figure S1. Photographs of tumors. Figure S2. Hematoxylin and eosin staining of sectioned heart, liver, spleen, lung, and kidney tissues. Figure S3. Hemolysis assay. Figure S4. Routine blood examination. Figure S5. Apoptosis assay in liver and spleen by western blot. Figure S6. Autophagy assay in liver and spleen by western blot. Figure S7. CD3+ T cells in mouse tumors by immunohistochemical staining. Figure S8. CD3+ T cells in mouse spleen by immunohistochemical staining. Figure S9. PD-L1 immunohistochemistry. Figure S10. Immune-memory effect and metastasis inhibition.

补充材料1：图S1：肿瘤实物照片。
图S2：心脏、肝脏、脾脏、肺脏及肾脏组织切片的苏木精-伊红（Hematoxylin and eosin）染色结果。
图S3：溶血实验（Hemolysis assay）。
图S4：血常规检测。
图S5：采用蛋白质印迹（Western blot）法检测肝脏与脾脏的细胞凋亡情况。
图S6：采用蛋白质印迹法检测肝脏与脾脏的细胞自噬情况。
图S7：免疫组织化学染色检测小鼠肿瘤组织中的CD3阳性T细胞。
图S8：免疫组织化学染色检测小鼠脾脏组织中的CD3阳性T细胞。
图S9：程序性死亡受体配体1（PD-L1）免疫组织化学染色。
图S10：免疫记忆效应与肿瘤转移抑制实验。