RNA-seq transcriptome analysis of HCMV (AD169) and mock infected MRC5 fibroblasts

We employed RNA-seq to map the transcriptome of human MRC5 fibroblasts during HCMV infection with AD169. These data will highlight the ways in which the HCMV infection alters RNA levels during infection. Infect quiescent MRC5 fibroblasts in 15-cm dishes at MOI = 3 and harvest samples for RNA-seq at 48 hpi.

本研究采用RNA测序（RNA-seq）技术，绘制了AD169株人类巨细胞病毒（HCMV）感染人MRC5成纤维细胞过程中的转录组图谱。该数据集将阐明HCMV感染期间病毒调控宿主RNA表达水平的具体机制。实验具体流程为：以感染复数（Multiplicity of Infection, MOI）=3的感染量，接种15cm培养皿中的静息态MRC5成纤维细胞，并于感染后48小时（hours post infection, hpi）收集样本用于RNA测序。