Single cell transcriptomic profiling of large intestinal enteroendocrine cells in mice. Single cell transcriptomic profiling of large intestinal enteroendocrine cells in mice

We report the different enteroendocrine populations in the large intestine in mice based on their transcriptomics profile. Hormone, receptor and transcription factors are analysed for differential expression between the different cell clusters to give a comprehensive view of the different characterisitcs of the distal entereondocrine cells Overall design: Large intestine epithelium from a Neurod1-crexEYFP mouse was digested into single cells and sorted based on EYFP fluorescence to collect only the enteroendocrine cells. 3500 cells were collected and their transcriptome sequenced using the Chromium system (10x Genomics) and a Hiseq4000 sequencer. Analysis of their transcriptome was perfomed using the cellranger pipeline

本研究基于转录组学特征，报道了小鼠大肠内的不同肠内分泌细胞（enteroendocrine cell）群。本研究针对不同细胞簇间激素、受体及转录因子的差异表达展开分析，以全面解析远端肠内分泌细胞的各项特征。整体实验设计：取Neurod1-crexEYFP转基因小鼠的大肠上皮组织，消化为单个细胞后，基于EYFP荧光分选以仅收集肠内分泌细胞。最终收集3500个细胞，采用Chromium系统（10x Genomics）与Hiseq4000测序仪完成其转录组测序。转录组数据分析采用cellranger分析流程完成。