Mapping of a resistance gene for Albugo laibachii from the Arabidopsis accession Sf-2. Resistance to Albugo laibachii in Sf-2

In a cross of Sf-2xMAGIC329, resistance segregated 3:1 (R:S) in the F2 generation, suggesting a single gene/locus confers resistance. To map the gene responsible, 200 susceptible individuals from the F2 were collected and DNA extracted. This pooled "bulk susceptible" DNA was captured using the BrRenSeq v2 bait library to select for NLR encoding loci and sequenced on the MiSeq platform as one of a set of pooled barcoded samples (TGAC-ENQ-1124/PRO1067).

在Sf-2xMAGIC329的杂交组合中，F2代群体的抗病性以3:1（抗∶感）的比例分离，提示该抗病性由单个基因/基因座（locus）所控制。为定位该抗病基因，研究人员收集了F2代中的200个感病个体并提取其基因组DNA。将该感病混合池DNA利用BrRenSeq v2诱饵文库进行捕获，以筛选编码核苷酸结合富亮氨酸重复序列（NLR）的基因座，随后将其作为一组带条形码标记的混合样本（TGAC-ENQ-1124/PRO1067）之一，在MiSeq测序平台上完成测序。