Analysis of AEF activity in peripheral blood monocytes isolated from mice with AA-amyloid induced by AEF.

AA-amyloid was induced in nine mice (G1–G9) by an i.v. injection of 0.1 ml AEF with concomitant s.c. injection of 0.2 ml 1% silver nitrate day 1, 7, 14, 21 and 28 and the mice were sacrificed on day 35. The presence of amyloid in the spleen was verified by Congo red staining. Peripheral blood monocytes were isolated, sonicated and re-introduced into the blood circulation of new groups of healthy mice (H1–H8). These mice received inflammatory stimuli day 1, 7 and 14 and were sacrificed day 16. The presence of amyloid was analysed in spleen sections after Congo red staining. Mice in group H9 received sonicated monocytes without subsequent inflammatory stimuli and group H10 received monocytes isolated from untreated mice and subsequent inflammatory stimuli on day 1, 7 and 14 and were sacrificed day 16 (group H10).

本研究对9只小鼠（G1~G9）通过静脉注射（intravenous, i.v.）0.1 mL AEF，并于第1、7、14、21、28天联合皮下注射（subcutaneous, s.c.）0.2 mL 1%硝酸银，诱导AA型淀粉样变，最终于第35天处死所有小鼠。通过刚果红染色（Congo red staining）验证了小鼠脾脏内淀粉样蛋白的存在。分离外周血单核细胞（peripheral blood monocytes），经超声处理后重新输注至新一批健康小鼠的血液循环中，该批小鼠分为H1~H8组。这些小鼠于第1、7、14天接受炎症刺激，并于第16天处死，随后通过刚果红染色对其脾脏切片中的淀粉样蛋白进行检测分析。其中H9组小鼠仅接受超声处理后的单核细胞输注，未给予后续炎症刺激；H10组小鼠输注的单核细胞取自未处理的小鼠，并于第1、7、14天接受炎症刺激，最终于第16天处死。