Single-nucleus RNA-seq identifies one galanin neuronal subtype in mouse preoptic hypothalamus activated during recovery sleep after sleep deprivation

The preoptic area of hypothalamus plays an important role in sleep homeostasis. We used snRNA-seq to identify neurons involved in homeostatic sleep response. Overall design: We compared mice under high sleep pressure (recovery sleep following sleep deprivation) to mice under low sleep drive (undisturbed sleep toward the end of lights-on).

下丘脑视前区在睡眠稳态调控中发挥重要作用。我们采用单细胞核RNA测序（snRNA-seq）技术，筛选参与睡眠稳态应答的神经元。实验设计概述：我们将处于高睡眠压力状态（睡眠剥夺后给予恢复睡眠）的小鼠，与处于低睡眠驱动状态（光照期末期的无干扰睡眠小鼠）进行对比。