H4K16 acetylation marks active genes and enhancers of embryonic stem cells, but does not alter chromatin compaction [Agilent array]

We report that acetylation of H4K16 is a new marker of active enhancers and that some enhancers are marked by H3K4me1, MOF and H4K16ac but not by acetylated H3K27 or p300, suggesting that they are novel p300-independent regulatory elements. Gene expression in undifferentiated 46c (sox1-gfp) ES cells, and gfp FACS sorted cells after 5 days of differentiation to neural progenitor cells with NB27 and Neuro2 medium supplements. 3 biological replicates were performed for each sample.

本研究报道，组蛋白H4K16乙酰化是活性增强子的新型标记物；部分增强子可被H3K4单甲基化（H3K4me1）、MOF及H4K16乙酰化（H4K16ac）标记，但无法被组蛋白H3K27乙酰化或p300标记，提示此类增强子为不依赖p300的新型调控元件。本数据集包含未分化的46c（sox1-绿色荧光蛋白）胚胎干细胞（Embryonic Stem Cells, ES），以及采用NB27与Neuro2培养基添加剂诱导分化5天后得到的神经前体细胞的绿色荧光蛋白阳性荧光激活细胞分选（Fluorescence-Activated Cell Sorting, FACS）细胞的基因表达谱；所有样本均设置3次生物学重复。