DataSheet1_A novel approach to assessing the antioxidant and anti-diabetic potential of synthesized calcium carbonate nanoparticles using various extracts of Ailanthus altissima.PDF

Calcium carbonate nanoparticles (CaCO3) have been found to exhibit unique properties that show their potential to be used in various therapies. Green synthesis of CaCO3 has been progressively gaining ac-ceptance due to its cost-effectiveness and energy-efficient nature. In the current study, different extracts of Ailanthus altissima were used to synthesize the calcium carbonate nanoparticles the synthesis and characterization of CCNPs were confirmed by using Fourier Transform Infra-Red spectroscopy, UV-Vis spectroscopy, and Scanning Electron Microscopy (SEM). The antioxidant activities (hydrogen peroxide, phosphomolydbenum, and ferric reducing) of calcium carbonate nanoparticles were affirmed by a good range of percentages of inhibition against free radical scavenging. The antidebate assays of CCNPs were observed by in-vitro and in silico approaches in a range at various concentrations while maximum inhibition occurred. In conclusion, the current study depicted that conjugated CaCO3 with A. altissima has a good potential to cure oxidative stress and Type II diabetes and could be used in the future as biogenic nanomedicine for the treatment of other metabolic diseases.

碳酸钙纳米颗粒（Calcium carbonate nanoparticles, CaCO₃）已被发现具备独特性能，展现出在多种治疗领域中的应用潜力。碳酸钙的绿色合成因其成本低廉、节能环保的特性，正逐渐获得广泛认可。本研究采用臭椿（Ailanthus altissima）的不同提取物制备碳酸钙纳米颗粒，并通过傅里叶变换红外光谱（Fourier Transform Infra-Red spectroscopy, FTIR）、紫外-可见光谱（UV-Vis spectroscopy）以及扫描电子显微镜（Scanning Electron Microscopy, SEM）对该纳米颗粒的合成过程与表征结果进行了确证。碳酸钙纳米颗粒的抗氧化活性涵盖过氧化氢法、磷钼酸法与铁还原法三种检测体系，其对自由基清除的抑制率处于较高水平，验证了其抗氧化功效。本研究通过体外实验与计算机模拟（in silico）方法，在不同浓度梯度下对碳酸钙纳米颗粒的抗糖尿病活性进行了检测，结果显示其具备最高抑制活性。综上所述，本研究表明，结合臭椿提取物制备的碳酸钙纳米颗粒具备改善氧化应激与治疗II型糖尿病的良好潜力，未来可作为生物源纳米药物，用于其他代谢类疾病的治疗。