The Effects of Static Magnetic Fields on Human Embryonic Cells. Homo sapiens

This study provides a framework describing how magnetic exposure is transduced from the most-plausible molecular-level ‘biosensor’ (lipid membranes) to cell-level responses that include differentiation toward neural lineages. In addition, SMF provided a stimulus that uncovered new relationships – that exist even in the absence of magnetic fields – between gangliosides, the time dependent regulation of IL-6 signaling by these glycolipids, and the fate of embryonic cells. Overall design: Subconfluent (70%-80%) undifferentiated LVECs (passage 11) were resuspended at 500k cells in 10 ml culture medium (day 0) and cultured in the presence or absence of static magnetic field. Four conditions were investigated: A) Control (without exposure); B) One day exposure (Exposed on day 6 without recovery); C) Continuous exposure (day 2 to day 5) followed by one day recovery; D) Continuous exposure (day 2 to day 6) without recovery. Cells are harvested on day 7. mRNA was isolated from the cells and microarray analysis was done using the Affymetric Human Genome U133 2.0 Plus Chip.

本研究构建了一套阐释框架，用以说明磁暴露如何从最具合理性的分子级‘生物传感器’（脂质膜（lipid membranes））转导至细胞级响应，其中包括向神经谱系（neural lineages）的分化过程。此外，静态磁场（Static Magnetic Field, SMF）作为刺激手段，揭示了即便在无磁场条件下依然存在的新关联：涉及神经节苷脂（gangliosides）、这类糖脂（glycolipids）对白细胞介素6（Interleukin 6, IL-6）信号通路的时间依赖性调控，以及胚胎细胞的命运走向。

整体实验设计：将处于70%~80%亚汇合状态的未分化LVECs（第11代）以每10ml培养基重悬50万个细胞的密度制备，记为第0天，随后分别在有、无静态磁场的条件下培养。本次实验共设置4组实验条件：
A）对照组（无磁暴露）；
B）单日磁暴露组（于第6天施加暴露且无恢复期）；
C）持续暴露组（第2天至第5天施加暴露，随后设置1天恢复期）；
D）持续暴露组（第2天至第6天施加暴露且无恢复期）。
所有细胞均于第7天收获。随后从细胞中分离总mRNA，并使用Affymetrix人类基因组U133 2.0 Plus芯片（Affymetrix Human Genome U133 2.0 Plus Chip）完成微阵列分析。