five

Selfing.

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NIAID Data Ecosystem2026-03-14 收录
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https://figshare.com/articles/dataset/Selfing_/21998238
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We selfed two F2 panel clones (94 and 693) with a recombination event in the F-locus region to test the independence of the ABC supergene and the F locus, and to attempt additional fine mapping. README) Column descriptions for following tables. Collection) Selfing is performed by collecting ephippia (sexually produced resting eggs) and hatching them after a period of dormancy. Ephippia were collected across four dates in 2019 and 2020. Attachment tests) Selfed offspring were tested for resistance to attachment by Pasteuria ramosa genotypes C19, P15, and P21. Resistance to these genotypes is known to segregate in the QTL panel. Red and blue shading correspond to susceptible (attachment positive) and resistant (attachment negative) phenotypes, respectively. Summary) Summary table of resistance to each of the three P. ramosa genotypes. We inferred the genotype of each locus predicted to confer resistance to the parasite genotypes, according to the genetic model of resistance and assuming Mendelian segregation. (XLSX)

本研究对两个在F位点(F-locus)区域存在重组事件的F2群体克隆(编号94与693)开展自交实验,以验证ABC超基因(ABC supergene)与F位点的独立性,并尝试开展进一步的精细定位。 【自述文件(README)】下述表格的列项说明。 【采集流程】自交操作通过收集卵鞍(ephippia,即有性繁殖产生的休眠卵),并在经过一段休眠期后使其孵化完成。卵鞍样本于2019年与2020年的四个采样时间点完成采集。 【附着抗性检测】对自交后代进行抗性测试,以验证其对枝芽孢杆菌(Pasteuria ramosa)基因型C19、P15及P21的附着抗性。已知对上述基因型的抗性在QTL(数量性状基因座,Quantitative Trait Locus)群体中存在分离现象。图表中红色与蓝色填充分别对应易感(附着阳性)与抗性(附着阴性)表型。 【抗性汇总】本表格汇总了对三种枝芽孢杆菌基因型的抗性情况。本研究依据抗性遗传模型,并假设遵循孟德尔分离定律,推断出每个预测可赋予对该寄生虫基因型抗性的位点的基因型。 (XLSX格式)
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2023-02-02
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