Genome-wide occupancy of LSD1 in HEL human erythroleukemia cell line.

We examined the genome-wide distribution of LSD1 bound regions in HEL cells bya ChIP-seq analysis. We found that GATA family transcription factors co-operate with LSD1 to regulate metabolic genes. We also found that LSD1 binds to and represses CEBPA gene enhancer, resulting in the suppression of myeloid cell properties. Overall design: Examination of LSD1 / DNA interaction in HEL. Two biological replicates were analyzed.

本研究通过染色质免疫共沉淀测序（ChIP-seq）分析，解析了HEL细胞中赖氨酸特异性去甲基化酶1（LSD1）结合区域的全基因组分布模式。研究发现，GATA家族转录因子可与LSD1协同调控代谢基因。本研究同时发现，LSD1能够结合并抑制CEBPA基因的增强子区域，进而抑制髓系细胞的相关特性。实验设计：检测HEL细胞中LSD1与DNA的相互作用，共设置2次生物学重复并开展分析。