Peptidome profiling of ovarian cancer and non-cancer proximal fluids: ascites and blood sera

Ovarian cancer is the most lethal gynecological malignancy, because its early detection is difficult due to the absence of recognizable physical symptoms and a lack of sensitive screening methods. Despite a large number of proteomic studies of biological fluids from ovarian cancer patients, only a few biomarkers are used in clinical practice. Low molecular weight endogenous peptides more easily diffuse across endothelial barriers and can be more relevant biomarker candidates. In this current study, detailed peptidomic analysis of 26 ovarian cancer and 15 non-cancer samples of biological fluids (ascites and sera) were performed using TripleTOF 5600+ mass-spectrometer.

卵巢癌是致死率最高的妇科恶性肿瘤，由于缺乏明确的躯体症状且缺少灵敏的筛查手段，其早期检测难度极大。尽管针对卵巢癌患者的生物体液已开展了大量蛋白质组学研究（proteomic studies），但目前仅有少数生物标志物应用于临床实践。低分子量内源性肽更易透过内皮屏障，可作为更具临床相关性的候选生物标志物。本研究采用TripleTOF 5600+质谱仪，对26份卵巢癌患者与15份非癌受试者的生物体液样本（腹水与血清）开展了详尽的肽组学分析（peptidomic analysis）。