16s genomic sequencing mountain forest soil

In the Appalachian context, it is worth noting the potentially unique role of Robinia pseudoacacia(black locust) in multi-species restoration. R. pseudoacacia is a nitrogen fixing tree that is common in early secondary forests on former agricultural lands. In these successional contexts it plays a unique role in nutrient cycling and creating habitat for forest species (Fowler et al., 2015). In addition to fixing nitrogen and increasing levels in the soil, it has been shown to increase soil phosphorus through leaf and flower deposition (Lee et al., 2011). Its ability to cycle phosphorus is related to its ability to form mycorrhizal associations. This restoration potential has been less studied in Appalachia. (Wang et al., 2020; Xu et al., 2020). R. pseudoacacia's role in phosphorus cycling during early succession creates potential synergies with the STEPS Project. The study aims to promote forest restoration through soil microbial community resilience, which accelerates the adoption of forest production, protection, restoration, and afforestation in Appalachia.

在阿巴拉契亚地区的研究背景下，值得关注的是刺槐（Robinia pseudoacacia，black locust）在多物种修复中可能具备的独特作用。刺槐是一种固氮树种，广泛分布于曾作为农业用地的早期次生林生境中。在该类演替场景下，刺槐在养分循环以及为森林物种营造栖息生境方面发挥着独特作用（Fowler等，2015）。除固氮并提升土壤氮素水平外，研究证实刺槐还可通过叶片与花部凋落物增加土壤磷素含量（Lee等，2011）。刺槐的磷素循环能力与其形成菌根共生关系的能力息息相关。目前阿巴拉契亚地区针对该树种的修复潜力研究仍较为不足（Wang等，2020；Xu等，2020）。刺槐在早期演替过程中的磷素循环功能，与STEPS项目存在潜在协同效应。本研究旨在通过提升土壤微生物群落韧性以推动森林修复，进而加快阿巴拉契亚地区森林生产、保护、修复与造林工作的推广应用。