DataSheet1_Construction of a mouse model that can be used for tissue-specific EV screening and tracing in vivo.docx

Extracellular vesicles (EVs) play an important role in the communication between tissues and cells. However, it is difficult to screen and trace EVs secreted by specific tissues in vivo, which affects the functional study of EVs in certain tissues under pathophysiological conditions. In this study, a Cre-dependent CD63flag-EGFP co-expressed with mCherry protein system expressing mice was constructed, which can be used for the secretion, movement, and sorting of EVs from specific tissues in vivo. This mouse model is an ideal research tool for studying the secretion amount, target tissue, and functional molecule screening of EVs in specific tissues under different pathophysiological conditions. Moreover, it provides a new research method to clarify the mechanism of secreted EVs in the pathogenesis of the disease.

细胞外囊泡（Extracellular vesicles, EVs）在组织与细胞间的信号交流中发挥关键作用。然而，在活体中筛选并示踪特定组织分泌的细胞外囊泡存在较大难度，这限制了病理生理状态下特定组织来源细胞外囊泡的功能研究。本研究构建了一种携带有Cre重组酶依赖型CD63flag-EGFP与mCherry蛋白共表达系统的转基因小鼠模型，该模型可用于活体中特定组织来源细胞外囊泡的分泌、迁移与分选过程研究。此小鼠模型是研究不同病理生理状态下特定组织来源细胞外囊泡的分泌量、靶向组织及功能分子筛选的理想研究工具，同时也为阐明分泌型细胞外囊泡在疾病发生发展中的作用机制提供了全新的研究手段。