TNFα secreted from macrophages increases expression of prometastatic integrin αV in gastric cancer. TNFα secreted from macrophages increases expression of prometastatic integrin αV in gastric cancer

We performed RNA-Seq to confirm the changes in expression of genes in the relevant pathway in human gastric cancer cells (AGS cells) and AGS cells cocultured with macrophages (RAW264.7 cells). In the gene ontology enrichment analysis of the 160 genes that were increased in AGS cells cocultured with RAW264.7 cells, pathways related to immune response, cell adhesion, and cytokine response were enriched. Overall design: AGS cells cocultured with RAW264.7 cells for 24 hours. >>>Submitter states that raw data are not available because they were not provided nor retained by the sequencing company<<<

本研究通过RNA测序（RNA-Seq）技术，验证人胃癌细胞（AGS细胞）以及与巨噬细胞RAW264.7细胞共培养的AGS细胞内相关通路基因的表达变化。针对与RAW264.7细胞共培养的AGS细胞中上调表达的160个基因开展基因本体富集分析（Gene Ontology Enrichment Analysis），结果显示免疫应答、细胞黏附及细胞因子应答相关通路显著富集。实验整体设计：将AGS细胞与RAW264.7细胞共培养24小时。>>>提交者声明：因测序公司未提供且未留存原始数据，故原始数据无法获取<<<