Physicochemical Characterizations and Antioxidant Property of Copaiba Oil Loaded into SNEDDS Systems

Self-nanoemulsion drug delivery systems (SNEDDS) represent a potential carrier to improve solubility and bioavailability of health care products. In this sense, copaiba oil (CO) loaded on cosurfactant-free SNEDDS formulations (CO-SNEDDS) were obtained by using phase diagram performed on different concentrations of constituents (oil, surfactant and water). The CO-SNEDDS are composed of 1% (m/m) of oil phase, 20% (m/m) of Tween 80, and 79% (m/m) of double-distilled water. Specifically, varying the oil phase to 1% of CO affords the so-called SNECO system, and 1% of CO blended on a 1:1 ratio with coconut oil or sunflower oil affords, respectively, the SNECO-COO and SNECO-SO derivative systems. CO sample was authenticated by gas chromatography equipped with a flame ionization detector and the CO-SNEDDS samples were characterized by droplet particle size, zeta potential, polarized light microscopy, pH, conductivity, refractive index, rheological and surface tension analyses. According to the antioxidant tests reducing power and hydroxyl inhibiting formation, the findings displayed 47.89 and 72.92% for SNECO, 46.48 and 84.11% for SNECO-COO, and 52.46 and 74.48% for SNECO-SO, respectively. From now on CO-SNEDDS based systems become available as promising targets for novel medicinal investigations and also could be undertaken for the oral administration of poorly water soluble drugs.

自纳米乳药物递送系统（Self-nanoemulsion drug delivery systems, SNEDDS）是一类可用于提升保健品溶解度与生物利用度的潜在载体。基于此，本研究通过针对不同组分（油相、表面活性剂与水）浓度绘制相图，制备得到了不含助表面活性剂的载古巴香油（copaiba oil, CO）的SNEDDS制剂（下称CO-SNEDDS）。该CO-SNEDDS配方组成为：1%（m/m）油相、20%（m/m）吐温80（Tween 80）以及79%（m/m）双蒸水。
具体而言，当油相仅为1%的古巴香油时，所得体系被称为SNECO系统；若将1%的古巴香油分别与椰子油、葵花油以1:1的比例复配作为油相，则可分别得到SNECO-COO与SNECO-SO衍生体系。
古巴香油样品通过配备火焰离子化检测器的气相色谱法完成定性鉴定，CO-SNEDDS样品则通过液滴粒径、Zeta电位、偏光显微镜观测、pH值、电导率、折射率、流变学特性及表面张力分析完成表征。
针对还原力与羟基自由基抑制能力两项抗氧化指标开展检测后，结果显示：SNECO体系的两项抗氧化指标分别为47.89%与72.92%，SNECO-COO体系为46.48%与84.11%，SNECO-SO体系则分别为52.46%与74.48%。
综上，本研究所获CO-SNEDDS基递送系统可作为新型药物研究的潜在候选靶点，同时亦可用于口服递送水溶性较差的药物。