mod2.2-VC.BG3 ChIP-chip

modENCODE_submission_324 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determe the locations of 125 chromosomal proteins and histone modifications across the Drosophila melanogaster genome. The proteins and modifications under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Keywords: CHIP-chip EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Cell Line: ML-DmBG3-c2; Tissue: CNS-derived cell-line; Developmental Stage: third instar larval stage; Genotype: y v f mal; Sex: Unknown; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Cell Line ML-DmBG3-c2; Antibody mod2.2-VC

modENCODE_submission_324 本提交源自Gary Karpen的modENCODE项目。如需查看modENCODE项目的完整列表，请访问http://www.genome.gov/26524648。
项目目标：本研究旨在确定125种染色体蛋白与组蛋白修饰在黑腹果蝇（Drosophila melanogaster）基因组中的定位。所研究的蛋白及修饰参与DNA复制、基因表达、基因沉默与遗传等基础染色体功能。本研究将采用染色质免疫沉淀（Chromatin ImmunoPrecipitation, ChIP）结合基因组平铺芯片的实验方法。初期将使用三种细胞系与两个胚胎阶段的染色质开展定位分析，后续将把部分蛋白的分析拓展至另外四种动物组织/发育阶段。关于数据使用的条款与条件，请参阅http://www.genome.gov/27528022 及 http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf。
关键词：ChIP-chip
实验类型：ChIP-chip
生物样本来源：细胞系：ML-DmBG3-c2；组织：中枢神经系统（Central Nervous System）来源细胞系；发育阶段：三龄幼虫期；基因型：y v f mal；性别：未知
重复次数：4
实验因子：细胞系ML-DmBG3-c2；抗体：mod2.2-VC