The effect of auxin exposure on the adult Drosophila transcriptome. The effect of auxin exposure on the adult Drosophila transcriptome

Auxin is used to induce transgene expression or degrade proteins in Drosophila. Here we exposed three different genotypes to 0 mM or 10 mM auxin and measured changes in gene expression for both males and females. We found that auxin exposure significantly alters the transcriptome of adult Drosophila in all genotypes tested. Overall design: To test the affects of auxin on the transcriptome, we fed 4-5 day old adult Drosophila to 0 mM or 10 mM auxin for 48 hours. We then processed whole body samples for RNA extraction and sequencing.

生长素（Auxin）可用于在果蝇（Drosophila）中诱导转基因表达或降解蛋白质。本研究将三种不同基因型的果蝇分别暴露于0 mM或10 mM生长素环境中，并检测了雌雄果蝇的基因表达变化。结果发现，在所有受试基因型中，生长素暴露均可显著改变成年果蝇的转录组（transcriptome）。
实验总体设计：为探究生长素对果蝇转录组的影响，我们将4-5日龄的成年果蝇投喂含0 mM或10 mM生长素的饲料，持续处理48小时。随后收集全身体样本进行RNA提取（RNA extraction）与测序（sequencing）。