Solanaceae comparative gene expression profiling. Solanaceae comparative gene expression profiling

Six different Solanaceae species, Potato (Solanum tubersosum), Tomato (Lycopersicum esculentum), Pepper (Capsicum annuum), Tobacco (Nicotiana tabaccum), Petunia and Nicotiana benthiamana were grown at 25C, 16h light and 8h darkness. Mature leaves were harvested after 4-6 weeks. RNA was isolated using Qiagen RNeasy. Tomato, pepper, petunia, tobacco and N. benthamiana samples were hybridized against potato samples. Keywords: Solanaceae comparative gene expression profiling Overall design: Six different Solanaceae species, Potato (Solanum tubersosum), Tomato (Lycopersicum esculentum), Pepper (Capsicum annuum), Tobacco (Nicotiana tabaccum), Petunia and Nicotiana benthiamana were grown at 25C, 16h light and 8h darkness. Mature leaves were harvested after 4-6 weeks. RNA was isolated using Qiagen RNeasy. Tomato, pepper, petunia, tobacco and N. benthamiana samples were hybridized against potato samples.

本数据集包含6个不同的茄科（Solanaceae）物种，分别为马铃薯（Solanum tubersosum）、番茄（Lycopersicum esculentum）、辣椒（Capsicum annuum）、烟草（Nicotiana tabaccum）、矮牵牛（Petunia）以及本氏烟草（Nicotiana benthiamana）。所有供试材料均于25℃、16小时光照与8小时黑暗的培养条件下种植。待植株生长4-6周后，采集成熟叶片。采用Qiagen RNeasy试剂盒完成RNA提取。将番茄、辣椒、矮牵牛、烟草及N. benthiamana样品与马铃薯样品进行杂交。
关键词：茄科比较基因表达谱分析
整体实验设计：6个不同的茄科（Solanaceae）物种，即马铃薯（Solanum tubersosum）、番茄（Lycopersicum esculentum）、辣椒（Capsicum annuum）、烟草（Nicotiana tabaccum）、矮牵牛（Petunia）以及本氏烟草（Nicotiana benthiamana），均于25℃、16小时光照与8小时黑暗的环境中培养。植株生长4-6周后采集成熟叶片，使用Qiagen RNeasy试剂盒分离RNA。随后将番茄、辣椒、矮牵牛、烟草及N. benthiamana样品与马铃薯样品进行杂交。