Analysis of metabolic characteristics of peripheral blood mononuclear cells in patients with leukemia at the single cell level

We collected peripheral blood from leukemia patients at different stages, including those newly diagnosed as untreated, as well as those in remission on therapy, with a subset of samples including peripheral blood and bone marrow samples. First, mononuclear cells were isolated from the collected peripheral blood, followed by single-cell RNA sequencing and follow-up sequencing results for subgroup classification, focusing on changes such as glycolipid metabolism of B cells as well as T cells. The aim of this project was to explore the changes in metabolic remodeling in leukemia patients with the aid of single-cell technology to provide assistance in the diagnosis and treatment of the disease. Overall design: The focus of this study was to collect peripheral blood from leukemia patients at different stages of disease onset, followed by single-cell RNA sequencing of peripheral blood mononuclear cells, combined with clinicopathological and other relevant data, to investigate changes in metabolic remodeling in leukemia patients. This project is a study of observational nature and does not involve modification of the treatment regimen to cause adverse effects to patients.

本研究收集了不同疾病阶段的白血病患者外周血样本，包括初诊未治患者以及接受治疗后处于缓解期的患者，其中部分样本同时包含外周血与骨髓标本。首先从收集的外周血中分离外周血单个核细胞（peripheral blood mononuclear cells），随后进行单细胞RNA测序，并结合后续测序结果进行亚群分类，重点关注B细胞与T细胞的糖脂代谢等变化。本项目旨在借助单细胞技术探究白血病患者体内的代谢重塑变化，以期为该疾病的诊疗提供辅助支持。

研究整体设计：本研究核心为收集不同发病阶段的白血病患者外周血，对外周血单个核细胞开展单细胞RNA测序，并结合临床病理等相关数据，以探究白血病患者的代谢重塑变化。本项目属于观察性研究，不会通过修改患者治疗方案来引发不良影响。