ZNF676 and ZNF728 ChIP-exo in HEK293T cells. ZNF676 and ZNF728 ChIP-exo in HEK293T cells

Genome-wide binding profile of the primate-specific KRAB zinc finger proteins ZNF676 and ZNF728 in cell lines overexpressing the proteins. Overall design: Stable HEK293T cell lines overexpressing ZNF676 and ZNF728 were made by lentiviral vector transduction and selection. They were then fixed with formaldehyde and immunoprecipitation was performed with an anti-HA antibody. An exonuclease treatment was performed according to ChIP-exo protocol.

本数据集包含灵长类特异性KRAB锌指蛋白（KRAB zinc finger protein）ZNF676与ZNF728在过表达该类蛋白的细胞系中的全基因组结合谱。
实验设计概述：通过慢病毒载体转导与筛选，构建得到过表达ZNF676及ZNF728的稳定HEK293T细胞系；随后以甲醛固定细胞，使用抗HA抗体开展免疫沉淀实验，并依照ChIP-exo实验流程进行核酸外切酶处理。