Drosophila species HiC and RNA sequencing

We generated HiC data from whole female flies for 22 Drosophila species, for which ONT-sequencing-based genome assemblies are available, to scaffold these assemblies in order to achieve chromosome-level genome quality, together with 8 species with publicly available HiC data. We further produced RNA sequencing data from ovaries for these 30 species to annotate the scaffolded genome assemblies.

我们为22个已有基于ONT测序（ONT-sequencing）基因组组装结果的果蝇属（Drosophila）雌性整虫生成了Hi-C（HiC）数据，用于对这些组装结果进行支架构建，以获得染色体级别的基因组组装质量；同时纳入了8个已有公开Hi-C数据的果蝇物种。随后，我们为这共计30个物种的卵巢组织生成了RNA测序数据，用于注释已完成支架构建的基因组组装结果。