MouseLight Neuron AA1451

A vast neural tracing effort by a team of Janelia scientists has upped the number of fully-traced neurons in the mouse brain by a factor of 10. Researchers can now download and browse the data in three dimensions. Inside the mouse brain, individual neurons zigzag across hemispheres, embroider branching patterns and, researchers have now discovered, can even spool out spindly fibers up to 45 centimeters long. Scientists can see and explore these wandering neural traces in 3-D in the most detailed map of mouse brain wiring yet created. The map reconstructs the shape and position of more than 300 of the 70 million neurons in the mouse brain. Previous efforts to trace the path of individual neurons had topped out in the dozens. The selectively-labeled neurons were mapped in an iterative process with two-photon microscopy. The brain is sliced in 200-micron sections and a few dozen neurons are labeled at a time and imaged. Each brain imaged yields about 50 terabytes of data, each containing mapped neurons which can be browsed via the NeuronBrowser application at https://ml-neuronbrowser.janelia.org/. Researchers interested in the complete raw data set should contact Janelia to discuss obtaining it via hardware transfer.

珍利亚研究园区（Janelia）的科学家团队开展了一项大规模神经追踪项目，将小鼠大脑中已完成全追踪的神经元数量提升了10倍。研究人员如今可下载并三维浏览该数据集。
在小鼠大脑内部，单个神经元会在大脑半球间蜿蜒穿行，编织出复杂的分支形态；研究人员此次还发现，神经元甚至可延伸出长达45厘米的纤细纤维。科学家们可在迄今最精细的小鼠大脑连接图谱中，以三维视角观察与探索这些蜿蜒的神经追踪轨迹。
该图谱重构了小鼠大脑7000万个神经元中300余个的形态与位置。此前的神经元追踪研究最多仅能完成数十个神经元的路径追踪。
本次研究通过双光子显微镜（two-photon microscopy）的迭代成像流程，对选择性标记的神经元进行了绘制。研究中将大脑切成200微米厚的切片，每次仅标记并成像数十个神经元。
每完成一个大脑的成像可产生约50太字节的数据，其中包含已追踪完成的神经元信息，研究人员可通过NeuronBrowser应用访问https://ml-neuronbrowser.janelia.org/进行浏览查询。
若有研究人员需要完整的原始数据集，请联系珍利亚研究园区（Janelia）协商通过硬件转移的方式获取。