AUF-1 affected genes in human skin cells

This study aims to evaluate the effect of AUF-1 knokdown on the function and global gene expression in human skin cells. Cells with AUF1 silencing were compared with control cells by RNA-Seq. Cell senescence is a tightly controlled, programmed process. Although the mechanism of cell senescence is unclear, the concept that cell aging is an interaction results between enviomental and genetic factors has been accepted. Changes in the expression of related genes are the genetic basis of cell senescence. AUF1 gene has been proved that can recognize classical mRNA and regulated its expression level. These classical mRNA include those encoding cecle-regulating proteins, apoptotic regulators, cytokines and proto-oncogenes. Our study use overexpression and interference lentivirus of AUF1 to transfect WS1 cell to process cell transcriptome sequencing. Through the Bioinformatics analysis results, we want to see some different expressed genes related to cell cecle, cell apoptosis and so on. Our study can provide basis research to further expound AUF1 gene, play an important role of aging mechanism. Overall design: Overexpression vectors and interference vectors were constructed and transfected into WS1 cells respectively. After 48h culture, second-generation sequencing was arranged

本研究旨在评估AUF1基因敲低（knockdown）对人皮肤细胞功能及全局基因表达的影响。通过RNA测序（RNA-Seq）技术，将AUF1沉默细胞与对照细胞进行对比分析。细胞衰老是一个受严格调控的程序性过程，尽管其具体机制尚未明确，但“细胞衰老是环境与遗传因素相互作用的结果”这一观点已被广泛接受。相关基因的表达变化是细胞衰老的遗传基础。已有研究证实，AUF1基因能够识别经典mRNA并调控其表达水平，这类mRNA包括编码细胞周期调控蛋白、凋亡调控因子、细胞因子及原癌基因（proto-oncogenes）的转录本。本研究通过构建AUF1过表达与干扰慢病毒（lentivirus），转染WS1细胞并开展细胞转录组测序。借助生物信息学（Bioinformatics）分析，我们旨在筛选出与细胞周期、细胞凋亡等相关的差异表达基因，本研究可为进一步阐明AUF1基因在衰老机制中的关键作用提供基础研究依据。
整体实验设计：分别构建过表达载体与干扰载体，并将其转染至WS1细胞中。培养48小时后，开展第二代测序（second-generation sequencing）。