Concentration-response transcriptomic changes induced by silver nanoparticles (AgNPs) in hepatocytes derived from human induced pluripotent stem cells (iPSCs). Concentration-response transcriptomic changes induced by silver nanoparticles (AgNPs) in hepatocytes derived from human induced pluripotent stem cells (iPSCs)

Transcriptomic changes in iPSC-derived hepatocytes after exposure to varying concentrations of AgNPs (0.01-25 μg/ml) for 24 h. Overall design: The experiment was conducted in two batches with the fist batch (batch 1) covering a higher concentration range (0, 5.0, 10, 20, and 25 µg/ml) and the second batch (batch 2) covering a lower concentration range (0, 0.01, 0.05, 0.1, 0.5, 1.0, and 5.0 µg/ml). Three replicates were included for each concentration.

不同浓度纳米银颗粒（AgNPs，0.01~25 μg/ml）处理24小时后，诱导多能干细胞（iPSC）分化肝细胞的转录组表达变化。实验设计概况：实验分为两批次开展，第一批次（批次1）覆盖较高浓度梯度（0、5.0、10、20、25 μg/ml），第二批次（批次2）覆盖较低浓度梯度（0、0.01、0.05、0.1、0.5、1.0、5.0 μg/ml）；各浓度组均设置3次生物学重复。