miRNA high-throughput sequencing of follicular thyroid tumors

We report data obtaibed from high-throughput sequencing of small RNAs in 20 samples of follicular thyroid tumors. We analyzed a total of 4.7±1.5million reads per sample with 3 different pipelines. The main goal was to evaluate the usefulness of next generation sequencing in small RNA profiling and the concordance of its results with microarrays and qPCR. Additionally we verified published follicular thyroid tumor biomarkers in the set of our samples. Overall design: Small RNA expression profiling with High Throughput Sequencing of 20 thyroid tumor samples, performed on an Illumina HiScan-SQ.

本研究报道了20例滤泡型甲状腺肿瘤样本中小RNA（small RNAs）高通量测序（high-throughput sequencing）获取的数据。
本研究采用三种不同的生物信息学分析流程，对每个样本的4.7±1.5百万条测序读段开展分析。
本研究的主要目标为评估下一代测序（next generation sequencing）在小RNA表达谱分析中的应用价值，以及其检测结果与基因芯片、实时定量PCR（qPCR）的一致性。
此外，本研究还在本次样本队列中验证了已发表的滤泡型甲状腺肿瘤生物标志物。
整体实验设计：基于Illumina HiScan-SQ测序平台，对20例甲状腺肿瘤样本开展小RNA表达谱高通量测序。