Gene expression profiles of desmoid tumor cells treated with gamma-secretase inhibitor

Desmoid tumors (DTs) are rare mesenchymal monoclonal lesions that have a high risk of local recurrence but lack metastatic potential. Since the Notch pathway appears to be important in the carcinogenesis of several tumor types, in the past few years γ-secretase inhibitors (GSIs) have emerged as a potential therapeutic treatment by inhibiting cancer cell Notch signaling through NICD cleavage blockade. To investigate the antitumor effect of PF-03084014, a γ-secretase inhibitor, in DT models, cells treated with PF-03084014 were characterized by gene array analysis. Three cell strains of Desmoid tumors (Desm14, Desm27 and Desm39b) were subjected to either no treatment (control, called G-0) or treatment with 10μM of PF-03084014 (called G-10). The regimen for each treatment was ~8 days and ~30 days; multiple group comparison

韧带样纤维瘤（Desmoid Tumors，DTs）是一类罕见的间质性单克隆病变，局部复发风险较高，但无转移潜能。由于Notch信号通路在多种肿瘤的发生发展中具有关键作用，近年来γ-分泌酶抑制剂（γ-secretase inhibitors，GSIs）通过阻断NICD切割以抑制癌细胞的Notch信号通路，成为潜在的抗肿瘤治疗手段。为探究PF-03084014（一种γ-分泌酶抑制剂）在韧带样纤维瘤模型中的抗肿瘤效应，研究人员对经PF-03084014处理的细胞开展了基因芯片分析。本研究选用3株韧带样纤维瘤细胞株（Desm14、Desm27与Desm39b），分别设置未处理对照组（记为G-0）与10μM PF-03084014处理组（记为G-10）。各组处理周期分别约为8天与30天；多组比较