Subgingival microbiota of 30 Japanese healthy or periodontitis adults. human oral metagenome

Gingival crevicular fluid samples of 30 Japanese adults were collected (Ikeda et al.). The 16S rRNA gene hypervariable V1-V2 regions were PCR-amplified using universal primers (a barcoded 27Fmod and 338R) and, multiplexed amplicon pyrosequencing was carried out using 454 GS FLX Titanium or 454 GS JUNIOR (Roche Applied Science).

本数据集包含池田等采集的30名日本成年人的龈沟液样本。研究人员采用带条形码标记的通用引物27Fmod与338R，对16S rRNA基因的高变V1-V2区域进行聚合酶链式反应（PCR）扩增，随后使用454 GS FLX Titanium或454 GS JUNIOR（罗氏应用科学公司）开展多重扩增子焦磷酸测序。