Microarray analysis of transcripts with elevated expressions in the rat medial or lateral habenula suggest fast GABAergic excitation in the medial habenula and habenular involvement in the regulation of feeding and energy balance

Full article is available in "Brain Structure and Function":http://link.springer.com/article/10.1007/s00429-016-1195-zby Franziska Wagner, René Bernard, Christian Derst, Leon French and Rüdiger W. Veh.In vertebrates the “anti-reward-system” mainly is represented by the habenula and its medial (MHb) and especially lateral (LHb) complexes. So far, considerable knowledge has accumulated especially concerning subnuclear structures and the connectivities of MHb and LHb subnuclei. The present investigation aimed to obtain novel information, whether MHb or LHb or their subnuclei display characteristic gene products, which may shed more light on biological functions of these areas. Using microarray analysis of mRNAs expressed in microdissected habenular and thalamic control areas yielded expression values of 17,745 RNAs representing protein-coding genes, to which annotated gene names could be assigned. High relative values of genes with known expression in MHb, LHb or thalamus in the corresponding areas indicated a high precision of the microdissection procedure. File descriptions Supplemental Table 1: Expression matrix with gene symbols and normalized values for each ref-seq probe. Supplemental Table 2: The first 100 transcripts with the highest absolute expression in MHb and LHb.Supplemental Table 3: Transcripts with enhanced expression in the lateral habenula as compared to the thalamus (FDR 1.5). A “+” in column J or K indicates that this gene also is included in the indicated table and correspondingly depicted in the intersections of Figure 1. Gene ontology biological process terms are listed in the last column.Supplemental Table 4: Transcripts with enhanced expression in the lateral habenula as compared to the thalamus (FDR 1.5). A “+” in column J or K indicates that this gene also is included in the indicated table and correspondingly depicted in the intersections of Figure 1. Gene ontology biological process terms are listed in the last column.Supplemental Table 5: Genes deferentially expressed between medial and the lateral habenula (FDR 1.5). A “+” in column J or K indicates that this gene also is included in the indicated table and correspondingly depicted in the intersections of Figure 1. Gene ontology biological process terms are listed in the last column.Supplemental Table 6: Gene ontology term enrichment results (six sheets in one table). These results were computed by ErmineJ. Gene Ontology files were downloaded on June 2015. Enrichment was tested on genes that had a corrected p

完整论文可参阅期刊"Brain Structure and Function"：http://link.springer.com/article/10.1007/s00429-016-1195-z，作者为Franziska Wagner、René Bernard、Christian Derst、Leon French及Rüdiger W. Veh。

在脊椎动物中，"反奖赏系统（anti-reward-system）"主要由缰核（habenula）及其内侧复合体（medial habenula, MHb），尤其是外侧复合体（lateral habenula, LHb）所构成。迄今为止，学界已积累了大量关于MHb与LHb的亚核结构及其连接特性的研究成果。本研究旨在获取全新数据，以明确MHb、LHb及其亚核是否存在特征性基因产物，进而为阐明这些脑区的生物学功能提供更多依据。

本研究通过对显微切割获取的缰核与丘脑对照脑区的mRNA进行微阵列分析，共得到17745个编码蛋白质基因的RNA表达值，且可为其分配已注释的基因名称。在对应脑区中，已知在MHb、LHb或丘脑内表达的基因呈现较高相对表达水平，这表明本次显微切割操作具有较高精准度。

### 文件说明
补充表1：包含基因符号与每个参考序列（ref-seq）探针标准化表达值的表达矩阵。
补充表2：在MHb与LHb中绝对表达量最高的前100个转录本。
补充表3：与丘脑相比，外侧缰核中表达上调的转录本（错误发现率FDR=1.5）。若J列或K列标注"+"，则表示该基因同时被纳入对应表格，并在图1的交集区域中相应展示。最后一列列出了基因本体（Gene Ontology, GO）的生物过程术语。
补充表4：与丘脑相比，外侧缰核中表达上调的转录本（FDR=1.5）。若J列或K列标注"+"，则表示该基因同时被纳入对应表格，并在图1的交集区域中相应展示。最后一列列出了GO生物过程术语。
补充表5：内侧缰核与外侧缰核之间差异表达的基因（FDR=1.5）。若J列或K列标注"+"，则表示该基因同时被纳入对应表格，并在图1的交集区域中相应展示。最后一列列出了GO生物过程术语。
补充表6：基因本体术语富集分析结果（单表内包含6个工作表）。该分析结果由ErmineJ软件计算得出。基因本体文件下载于2015年6月。富集分析针对带有校正后p值的基因。