Paratydeidae Scolotydaeus vlaskini k-1462 tarsus II ventral
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Original image stacks for descriptions of fossil mites of the family Paratydeidae from the late Eocene Rovno Amber. <br>Two species: <br>Scolotydaeus vlaskini n. sp.Housed in the amber collection of the Sсhmalhausen Institute of zoology NAS of Ukraine, Kiev, with collection number SIZK K‑1462 and Tanytydeus pogrebnyaki n. sp.<br>Housed in the amber collection of the Schmalhausen Institute of zoology NAS of Ukraine, Kiev, with collection number SIZK K‑18364. Compound microscopes Nikon E-800 with water immersion optics (40x and 60x) and Zeiss AxioImager A2 with dry (40x, 63x) and oil immersion optics (100x). Brightfield, polarized and differential interference contrast and incident illumination were used. Stacks of images, comprising multiple focal planes, were obtained with digital cameras Olympus OM-D E-M10II on Nikon microscope, Zeiss Axiocam 506 color and Hitachi KP-HD20A on Zeiss microscope. Stacks were corrected for colour, brightness and noise with Adobe Lightroom, then exported to JPEG format. <br>Combined images prepared with Helicon Focus 6.8.0, using algorithms A (mostly) and B with subsequent manual addition of significant details from the individual focal planes.
晚始新世罗夫诺琥珀(Rovno Amber)中副绥螨科(Paratydeidae)化石螨类形态描述配套的原始图像堆栈。本次研究涉及两个物种:Scolotydaeus vlaskini 新种(n. sp.):标本保存于乌克兰基辅国家科学院施马尔豪森动物学研究所琥珀馆藏,馆藏编号为SIZK K‑1462;Tanytydeus pogrebnyaki 新种(n. sp.):标本保存于乌克兰基辅国家科学院施马尔豪森动物学研究所琥珀馆藏,馆藏编号为SIZK K‑18364。成像所用复合显微镜包括:配备水浸物镜(40×、60×)的尼康(Nikon)E-800,以及搭载干式物镜(40×、63×)与油浸物镜(100×)的蔡司(Zeiss)AxioImager A2。成像模式涵盖明场照明、偏振光照明、微分干涉差(DIC)照明及落射照明。通过搭载于尼康显微镜的奥林巴斯(Olympus)OM-D E-M10II数码相机,以及搭载于蔡司显微镜的蔡司Axiocam 506 color数码相机与日立(Hitachi)KP-HD20A数码相机,采集包含多个焦平面的图像堆栈。图像堆栈经Adobe Lightroom软件完成色彩、亮度与噪声校正后,导出为JPEG格式文件。最终合成图像通过Helicon Focus 6.8.0软件制作完成,主要采用算法A、辅以算法B,并通过手动补充单个焦平面中的关键细节以优化图像效果。
创建时间:
2019-04-06



