RNA Sequencing for reprogrammed beta cells

Alpha-cell-derived INS+ cells (yellow cells) were isolated 1 month after AAV-PM (adeno-associated virus carrying pdx1 and mafA under the CMV promoter) infusion from ALX-treated GCG-Cre; R26RTomato; MIP-GFP mice, based on expression of tomato red (alpha-cell lineage) and GFP by flow cytometry. Sorted normal GFP+ beta cells and normal TOM+ alpha cells from GCG-Cre; R26RTomato; MIP-GFP mice without any treatment were used as controls. Overall design: Examination of gene profile in reprogrammed mouse beta cells from alpha cells, compared to primary mouse beta cells and alpha cells

从经ALX处理的GCG-Cre；R26RTomato；MIP-GFP小鼠中，于AAV-PM（携带CMV启动子驱动pdx1与mafA基因的腺相关病毒）输注后1个月，通过流式细胞术（flow cytometry）基于番茄红（α细胞谱系标记）与GFP的表达特征，分选得到α细胞来源的INS+细胞（黄色细胞）。将未经任何处理的GCG-Cre；R26RTomato；MIP-GFP小鼠中分选获得的正常GFP+ β细胞与正常TOM+ α细胞作为对照样本。实验整体设计：对比分析α细胞重编程来源的小鼠β细胞、原代小鼠β细胞与原代小鼠α细胞的基因表达谱。