Supplementary Figure 25 Transmission Electron Microscopy (TEM) depicts fractal-like assemblies in the phosphorylated samples while the non-phosphorylated samples depict individual proteins

<b>Transmission Electron Microscopy (TEM) depicts fractal-like assemblies in the phosphorylated samples while the non-phosphorylated samples depict individual proteins.</b> (<b>A </b>and <b>B</b>) ten-fold dilution of 3 µM non-pY-AtzAM1 and 2 µM AtzCM1, which shows the individual proteins. (<b>C </b>to <b>F</b>) Various assembly images of the ten-fold dilution of 3 µM pY-AtzAM1 and 2 µM AtzCM1 sample which form the fractal-like assembly consistently. (<b>G</b>) Image analysis (2D) using box counting yields the expected fractal dimension of ~1.7 for the C, D, and E, TEM images.

透射电子显微镜（Transmission Electron Microscopy, TEM）可观测到磷酸化样品中存在类分形组装体，而非磷酸化样品则呈现单个蛋白质分子。（图A和图B）为3 μM 非pY-AtzAM1与2 μM AtzCM1的10倍稀释样品，呈现出单个蛋白质分子的形态。（图C至图F）为3 μM pY-AtzAM1与2 μM AtzCM1的10倍稀释样品的各类组装图像，该样品始终形成类分形组装体。（图G）通过盒计数法开展二维图像分析，结果显示图C、D、E的透射电子显微镜图像的分形维数约为1.7，与预期值相符。