Hippophae rhamnoides subsp. sinensis Transcriptome or Gene expression

Illumina HiSeq high-throughput sequencing technology was applied to get the transcriptome from leaves of female and male plants leaves in Hippophae rhamnoides L. subsp. sinensis. Via sequencing, 48.31 Gb effective data was obtained with an average error rate of 0.015%. After de novo assembly by the software of Trinity, a total of 320876 unigenes and 187362 transcripts were generated, accounting for maximum length of 17291 bp, the average length of 588 bp and 808 bp respectively. Using Blastx against the public databases, 104926 unigene were annotated in at least one databases. In addition, 33248 potential SSR loci were detected throughout the transcriptomic data. 92970 genes showed differences in expression levels between the transcriptome of female and male plants leaves of Hippophae rhamnoides L. subsp. sinensis. This work provides basic data for the gene cloning and gene mining, as well as to investigate the sex-determining mechanism of dioecious Hippophae rhamnoides L. subsp. sinensis.本研究采用Illumina HiSeq高通量测序技术，获取中国沙棘（Hippophae rhamnoides L. subsp. sinensis）雌雄植株叶片的转录组。经测序，共获得48.31 Gb有效数据，平均错误率为0.015%。使用Trinity软件进行从头组装后，共得到320876个单基因簇（unigene）与187362条转录本，二者的最大长度分别为17291 bp，平均长度分别为588 bp与808 bp。通过Blastx比对公共数据库，共有104926个单基因簇至少在一个数据库中获得功能注释。此外，在全部转录组数据中共检测到33248个潜在的简单序列重复（Simple Sequence Repeat，SSR）位点。中国沙棘雌雄植株叶片转录组间共有92970个基因存在表达量差异。本研究为该雌雄异株中国沙棘亚种的基因克隆、基因挖掘以及解析其性别决定机制提供了基础数据。