Whole-genome gene expression profiles of non-tumorous human lung tissues: Laval set

We profiled genome-wide gene expression in non-tumorous human lung tissues. The overall goal of this project is to improve our molecular understanding of various lung diseases including lung cancer and chronic obstructive pulmonary disease (COPD). Lung specimens were obtained from patients undergoing lung cancer surgery at the Institut Universitaire de Cardiologie et de Pneumologie de Québec. All lung specimens were derived from a homogeneous piece of peripheral tissue free of tumour obtained at surgery performed between April 2004 and December 2008. These lung tissues were stored in the biobank of the Respiratory Health Network of the FRSQ, site Institut Universitaire de Cardiologie et de Pneumologie de Québec. The time from surgical removal to freezing was between 15 to 30 minutes. RNAs were extracted and then hybridized to a single custom-made whole-genome human Affymetrix array.

本研究对非肿瘤性人类肺组织进行了全基因组基因表达谱分析。本项目的核心目标是加深对包括肺癌、慢性阻塞性肺疾病（COPD）在内的多种肺部疾病的分子层面认知。肺标本采集自在魁北克大学心血管与肺病研究所（Institut Universitaire de Cardiologie et de Pneumologie de Québec）接受肺癌手术的患者。所有肺标本均取自2004年4月至2008年12月间手术中获取的、无肿瘤的均质外周肺组织块。上述肺组织被保存于魁北克省卫生研究基金（FRSQ）呼吸健康网络的生物样本库，该样本库设于魁北克大学心血管与肺病研究所。样本从手术切除至冻存的时间间隔为15至30分钟。随后提取RNA，并将其与单张定制化全基因组人类Affymetrix基因芯片进行杂交。