S-phase enriched non-coding RNAs regulate gene expression and cell cycle progression

We infer that non-coding RNAs can function as key effectors during cell cycle, acting on distinct regulatory networks to ensure maintenance of gene expression programs and cell cycle progression. We performed nascent RNA-seq in asynchronized RPE-1 cells as well as synchronized RPE-1 cells at five different timepoints of S-phase of cell cycle progression. We also performed strand-specific RNAseq on control and three knockdown cell lines in which a lincRNA of interest was knocked down. We also performed histone ChIP-seq and Cut-n-run seq on asynchronized RPE1 cells.

本研究推测，非编码RNA（non-coding RNAs）可作为细胞周期进程中的关键效应分子，通过作用于不同调控网络以维持基因表达程序稳定、保障细胞周期顺利推进。本研究对未同步化的RPE-1细胞，以及处于细胞周期S期五个不同时间节点的同步化RPE-1细胞，开展了新生RNA测序（nascent RNA-seq）实验；同时针对对照组及3株敲降目标基因间长链非编码RNA（lincRNA）的细胞系，完成了链特异性RNA测序（strand-specific RNAseq）；此外还对未同步化的RPE-1细胞进行了组蛋白染色质免疫共沉淀测序（histone ChIP-seq）与Cut-n-run测序（Cut-n-run seq）实验。