Transcriptome sequencing for cancer stem cell properties in MCF-7 cells with knocked down CD24. Homo sapiens

CD24 is the one of cell surface protein that anchored via glycosyl phosphatidylinositol that links to cell surface and modulates growth and differentiation signal many of cell types. A small population of cells, namely ‘Cancer Stem Cells (CSCs)’, charges highly aggressive and metastatic character of cancer cells and shows conformational change of ‘epithelial to mesenchymal transition (EMT). To understand the role of CD24 in CSC and EMT, CD24 was knocked down using siRNA in the MCF7 cell line (MCF-7 hCD24 KD) and analyzed transcriptome profiles by mRNA-sequencing. Corresponding author: Chul Geun Kim, Department of Life Science, Hanyang University, Seoul 133-791, Korea (e-mail, [e-mail]). Overall design: mRNA profiles of MCF-7 hCD24 KD cells [‘monolayer (m) cultured MCF7 wild type (WT) and mMCF7 CD24KD’ and ‘sphere-forming (s) MCF7 WT and sMCF7 CD24KD’] were generated by deep sequencing using Illumina GAIIx.

CD24是一类通过糖基磷脂酰肌醇（glycosyl phosphatidylinositol）锚定并结合于细胞表面的膜蛋白，可调控多种细胞类型的生长与分化信号通路。少量被称为"癌症干细胞（Cancer Stem Cells, CSCs）"的细胞群体，可赋予癌细胞高度侵袭与转移的特性，并发生"上皮间质转化（epithelial to mesenchymal transition, EMT）"的构象变化。为阐明CD24在癌症干细胞与上皮间质转化过程中的作用，研究人员通过小干扰RNA（small interfering RNA, siRNA）在MCF7细胞系中敲低CD24的表达（构建MCF-7 hCD24 KD细胞），并利用mRNA测序技术分析其转录组特征。通讯作者：金哲根（Chul Geun Kim），汉阳大学生命科学系，韩国首尔133-791（电子邮箱：[e-mail]）。实验整体设计：通过Illumina GAIIx平台开展深度测序，获取四组样本的mRNA表达谱：单层培养的MCF7野生型（WT）细胞、单层培养的MCF7 CD24敲低细胞，以及成球培养的MCF7野生型细胞、成球培养的MCF7 CD24敲低细胞。