Microbial data from the Macquarie Island Respirometry Experiment 2006

A microcosm experiment utilising a respirometry system and 14C-labelled hexadecane was initiated to investigate the effects of differing oxygen regimes on hydrocarbon degradation in soil from sub-Antarctic Macquarie Island. Measurements of oxygen consumed, carbon dioxide produced, total petroleum hydrocarbon degradation and nitrate and ammonium concentrations were made. The microbial community structure at the start of the experiment and after 4, 8 and 12 weeks incubation was explored using terminal restriction fragment length polymorphism and real-time PCR quantification of alkane mono-oxygenase, napthalene dioxygenase, nitrous oxide reductase and ribosomal polymerase sub-unitB. The data described here are the microbial community data only.The download file contains an excel spreadsheet. The first sheet provides further information about the dataset.This work was part of AAS projects 2672 and 1163.

本研究开展微宇宙实验，采用呼吸测量系统与碳14（¹⁴C）标记的十六烷，旨在探究不同氧状态对亚南极麦夸里岛土壤中烃类降解过程的影响。实验中测定了耗氧量、二氧化碳生成量、总石油烃降解率以及硝酸盐与铵盐浓度。实验起始时以及培养4、8、12周后，分别采用末端限制性片段长度多态性技术，以及针对烷烃单加氧酶、萘双加氧酶、一氧化二氮还原酶与核糖体聚合酶亚基B的实时定量PCR，对微生物群落结构进行解析。本数据集仅包含微生物群落相关检测数据。下载文件内含Excel电子表格，其第一张工作表包含本数据集的补充说明信息。本研究隶属于AAS项目2672与1163。