Determination of in-vitro phenolics, antioxidant capacity and bio-accessibility of Kombucha tea produced from black carrot varieties grown in Turkey

Abstract Black carrot, which is an economically important product, is produced extensively in Konya and Hatay regions in Turkey. Kombucha tea is a symbiotic system, comprises of bacteria and yeasts cultures called as SCOBY, and produced by fermentation of sugar and tea leaves. Kombucha samples contained black carrot juice in two concentrations (10 and 20%) and compared with the Kombucha contained green tea leaves. Black carrot varieties were used for enrichment of Kombucha production and investigated in terms of physico-chemical properties and antioxidant capacity. Black carrot samples belonging to the Hatay region, have higher anthocyanin content (660.26 mg C3G/100 g-dw), antioxidant capacity (TEACABTS and TEACCUPRAC; 15.33 ± 0.39 and 21.91 ± 0.28 µmole Trolox/g), total phenolic content (67.22 ± 0.24 mg/g GAE) and bioaccessibility (36.48 ± 0.78 mg/g GAE) comparing to Hatay black carrot variety. The highest anthocyanin content (71.05 mg C3G/100 mL), antioxidant capacity (TEACABTS and TEACCUPRAC; 3.67 ± 0.15 and 12.33 ± 0.11 µmole Trolox/g) were obtained from Kombucha tea containing black carrot juice (20%). According to the sensorial evaluation, panelists stated that the Kombucha samples with Hatay Black carrot juice had a fresh and pleasant taste and flavor due to green tea aroma.

黑胡萝卜是一类经济价值突出的农产品，在土耳其科尼亚（Konya）与哈塔伊（Hatay）两大产区有着广泛的种植规模。康普茶（Kombucha tea）是一种共生体系，由被称为SCOBY的细菌与酵母菌菌群构成，通过蔗糖与茶叶的发酵过程制备得到。本研究设置了添加10%与20%两种浓度黑胡萝卜汁的康普茶样品，并以纯绿茶康普茶作为对照进行对比分析。研究通过引入不同黑胡萝卜品种来强化康普茶的制备，并对所得样品的理化特性与抗氧化能力展开了表征分析。来自哈塔伊产区的黑胡萝卜样品，其花青素含量（660.26 mg C3G/100 g干重）、抗氧化能力（ABTS法总抗氧化能力TEACABTS与CUPRAC法总抗氧化能力TEACCUPRAC分别为15.33±0.39与21.91±0.28 μmol Trolox/g）、总酚含量（67.22±0.24 mg/g GAE）与生物可及性（36.48±0.78 mg/g GAE）均高于科尼亚产区的黑胡萝卜品种。添加20%黑胡萝卜汁的康普茶样品，其花青素含量（71.05 mg C3G/100 mL）与抗氧化能力（ABTS法总抗氧化能力TEACABTS与CUPRAC法总抗氧化能力TEACCUPRAC分别为3.67±0.15与12.33±0.11 μmol Trolox/g）为所有样品中的最高值。感官评价结果显示，品鉴小组认为添加哈塔伊黑胡萝卜汁的康普茶样品，因带有绿茶香气而具备清新宜人的口感与风味。