Super enhancers orchestrate osteogenesis program through navigation and initiation effects of Brd4 on Zbtb16 [CUT&TAG]

We performed multiomic high-throughput sequencing to recapitulated the primary mechanism of mesenchymal stem cells osteogenesis. We analysed the H3K27ac ChIP-seq, BRD4 CUT&Tag and RNA-seq data before and after osteogenesis, and identified super enhancers (SEs) regulated osteogenic identity genes in MSCs. Overall design: Examination of the BRD4 binding to genome in MSCs

本研究采用多组学高通量测序技术，解析间充质干细胞（mesenchymal stem cells，MSCs）成骨分化的核心机制。我们分析了成骨分化前后的H3K27乙酰化染色质免疫共沉淀测序（H3K27ac ChIP-seq）、BRD4靶向切割与标签化测序（BRD4 CUT&Tag）以及RNA测序（RNA-seq）数据，并鉴定出间充质干细胞中调控成骨特性基因的超级增强子（super enhancers，SEs）。整体实验设计：检测间充质干细胞内BRD4与基因组的结合情况。