Modified technique for preparation of venous circulation resin casts in the cirrhotic liver

ABSTRACT This study describes two major adaptations for the preparation of resin casts in human cirrhotic liver, harvested at the time of transplantation. The first is the way of fixing the catheter in the ostia of the hepatic and portal veins through a cerclage, so as to prevent displacement of the catheter and / or leakage of the resin during its injection. The second is the extension of corrosion time in the NaOH solution, averaging 6.8 days, with daily replacement the solution until complete removal of parenchymal tissue. We applied the method in 14 cirrhotic livers, with good filling and coloring of the portal and hepatic vein territories, using different colors. This allows an anatomical study of these vessels, able to complement the knowledge of the histopathology in research work, and the planning of therapeutic procedures, such as the Trans-Jugular Intrahepatic Port-Systemic Shunt (TIPS).

摘要：本研究针对移植术中获取的人类肝硬化肝脏的树脂铸型制备方法，提出两项核心改进措施。第一项改进为通过环扎术将导管固定于肝静脉与门静脉的血管开口处，以避免树脂注射过程中导管移位或树脂渗漏。第二项改进为延长氢氧化钠（NaOH）溶液的腐蚀时长，平均时长为6.8天，期间每日更换溶液直至肝实质组织完全清除。我们将该方法应用于14例肝硬化肝脏样本，通过使用不同着色剂实现了门静脉与肝静脉灌注区域的良好充盈与着色。该方案可支持上述血管的解剖学研究，辅助完善相关研究中的组织病理学认知，同时可为经颈静脉肝内门体分流术（Trans-Jugular Intrahepatic Port-Systemic Shunt, TIPS）等治疗操作的术前规划提供参考依据。