Expression data of Mesenchymal Stroma Cells (MSC)-adherent and non-adherent, human cord blood-derived, hematopoietic CD34 cells

MSC-adherent hematopoietic stem and progenotir cells (HSPC) express adhesion-associated genes at higher levels than non-adherent cells while cell-cycle and differentiation-associated genes are not significantly changed between the two cell populations. We used microarray to confirm identity of MSC-adherent and non-adherent cord blood-derived HSPCs and to exclude that cell cycle and differentiation affect adhesive capacity. CD34 positive cells were isolated from human cord blood (not older than 24h), expanded for 3 days and assayed for the adhesion to MSC. The adherent and non-adherent live CD34+ cells were sorted and total RNA was extracted.

黏附于间充质干细胞（Mesenchymal Stem Cell，MSC）的造血干祖细胞（hematopoietic stem and progenitor cells，HSPC），其黏附相关基因的表达水平高于非黏附类细胞；而两类细胞群体间与细胞周期、分化相关的基因表达无显著差异。本研究采用基因芯片（microarray）技术，对脐带血来源的黏附型与非黏附型HSPC进行身份验证，并排除细胞周期与分化过程对细胞黏附能力的影响。研究人员从采集时间不超过24小时的人脐带血中分离CD34阳性细胞，经3天体外扩增后，检测其与间充质干细胞的黏附能力。随后分选出存活的黏附型与非黏附型CD34阳性细胞，并提取总RNA。