Foxp3 depends on Ikaros for control of regulatory T cell gene expression and function [ATAC-Seq]

We investigated the role of Ikaros in regulatory Tcell identity and function during stimulation using RNA-seq, ATAC-seq, and H3K27ac/Foxp3/Ikzf1 ChIP-seq We performed ATAC-seq, RNA-seq, and ChIPseq against H3K27ac, Foxp3, and Ikzf1 on mouse Tregs

本研究借助RNA测序（RNA-seq）、转座酶可及性测序（ATAC-seq）以及组蛋白H3赖氨酸27乙酰化（H3K27ac）、叉头框P3（Foxp3）与Ikzf1的染色质免疫沉淀测序（ChIP-seq），探究了艾卡罗斯（Ikaros）在刺激条件下对调节性T细胞（regulatory T cell，Treg）特性与功能的调控作用，并以小鼠调节性T细胞为实验对象完成了上述所有测序实验。